A highly mutable GST is essential for bract colouration in Euphorbia pulcherrima Willd. Ex Klotsch
نویسندگان
چکیده
Abstract Background Mutation breeding is an extraordinary tool in plant to increase the genetic variability, where mutations anthocyanin biosynthesis are targets generate distinctive phenotypes ornamental species. In poinsettia, ionizing radiation routinely applied programs obtaining a range of colours, with nearly all pink and white varieties being obtained after γ- or X-ray mutagenesis red varieties. present study we performed thorough characterization potential target gene as main responsible for ‘ paradox ’ poinsettia. Results We identified GST poinsettia ( Bract1 ) essential factor expression anthocyanin-based colouration bracts, which presents high phylogenetic similarity known anthocyanin-related GSTs. Red mutants generated from these by were analysed polymorphisms related A 4 bp mutation short repeat within coding region most likely appearance upon irradiation treatment. The polymorphism between wild-type mutant alleles co-segregates phenotype progeny heterozygous parents. Moreover, overexpression allele Arabidopsis tt19 restored phenotype, while mutated showed be non-functional. Conclusions seems highly unstable, since plants can easily detected among fewer than 200 shoots derived 10 plants. Our data indicate that particular sequences, similar microsatellite sequences so-called dynamic mutations, might hot spots variability. identification fills gap on understanding molecular mechanism colour formation
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ژورنال
عنوان ژورنال: BMC Genomics
سال: 2021
ISSN: ['1471-2164']
DOI: https://doi.org/10.1186/s12864-021-07527-z